human ccl2 elisa Search Results


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CCL20 and CCL24 involved in crizotinib resistance. A 10 chemokines mRNA expression in H3122CR compared with H3122 (Control); B 4 chemokines protein expression in H3122 and H3122CR cell supernatants; C The mRNA and protein expression of 4 chemokines after si-RNA treatment compared with si-negative control (si-NC); D–F Effect of <t>CCL2,</t> CCL20, CCL24, and CX3CL1 knockdown on (D) cell proliferation of H3122CR; (E) cell cycle distribution of H3122CR; F half maximal inhibitory concentration (IC50) of crizotinib in H3122CR. * p < 0.05, ** p < 0.01, *** p < 0.001, ns: not significant
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Boster Bio elisa kit
CCL20 and CCL24 involved in crizotinib resistance. A 10 chemokines mRNA expression in H3122CR compared with H3122 (Control); B 4 chemokines protein expression in H3122 and H3122CR cell supernatants; C The mRNA and protein expression of 4 chemokines after si-RNA treatment compared with si-negative control (si-NC); D–F Effect of <t>CCL2,</t> CCL20, CCL24, and CX3CL1 knockdown on (D) cell proliferation of H3122CR; (E) cell cycle distribution of H3122CR; F half maximal inhibitory concentration (IC50) of crizotinib in H3122CR. * p < 0.05, ** p < 0.01, *** p < 0.001, ns: not significant
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Image Search Results


CCL20 and CCL24 involved in crizotinib resistance. A 10 chemokines mRNA expression in H3122CR compared with H3122 (Control); B 4 chemokines protein expression in H3122 and H3122CR cell supernatants; C The mRNA and protein expression of 4 chemokines after si-RNA treatment compared with si-negative control (si-NC); D–F Effect of CCL2, CCL20, CCL24, and CX3CL1 knockdown on (D) cell proliferation of H3122CR; (E) cell cycle distribution of H3122CR; F half maximal inhibitory concentration (IC50) of crizotinib in H3122CR. * p < 0.05, ** p < 0.01, *** p < 0.001, ns: not significant

Journal: Journal of Translational Medicine

Article Title: Role of chemokine-mediated angiogenesis in resistance towards crizotinib and its reversal by anlotinib in EML4-ALK positive NSCLC

doi: 10.1186/s12967-022-03451-2

Figure Lengend Snippet: CCL20 and CCL24 involved in crizotinib resistance. A 10 chemokines mRNA expression in H3122CR compared with H3122 (Control); B 4 chemokines protein expression in H3122 and H3122CR cell supernatants; C The mRNA and protein expression of 4 chemokines after si-RNA treatment compared with si-negative control (si-NC); D–F Effect of CCL2, CCL20, CCL24, and CX3CL1 knockdown on (D) cell proliferation of H3122CR; (E) cell cycle distribution of H3122CR; F half maximal inhibitory concentration (IC50) of crizotinib in H3122CR. * p < 0.05, ** p < 0.01, *** p < 0.001, ns: not significant

Article Snippet: CCL2 (SinoBiological, KIT10134), CCL20 (Abcam, ab269562), and CCL24 (Abcam, ab10050) levels in culture supernatant after treatment for 48 h were measured using ELISA kits.

Techniques: Expressing, Negative Control, Concentration Assay

CCL20 may induce crizotinib resistance by activation of angiogenesis via JAK2/STAT3-CCL20-VEGFA/IL6 axis. A a) Tube formation of HUVECs cultured in H3122 culture medium (CM), H3122CR CM, H3122 CM supplemented with human recombinant CCL20, and H3122 CM supplemented with rhCCL24. b) Tube formation of HUVECs cultured in the CM of H3122CR transfected with si-negative control (si-NC), si-CCL20, and si-CCL24. The number of tubes and capillary length were analyzed to evaluate angiogenic activity of CCL20 and CCL24; B , C Knockdown of CCL20 suppressed the protein expression of CCL2, IL6, and VEGFA in H3122CR compared with si-NC (Control); D STAT3 was upregulated in H3122CR compared with H3122; E , F Stattic inhibited the JAK2/STAT3 pathway and the protein expression of CCL20 and VEGFA in H3122CR. GAPDH served as a loading control. *p < 0.05, **p < 0.01, ***p < 0.001, ns not significant

Journal: Journal of Translational Medicine

Article Title: Role of chemokine-mediated angiogenesis in resistance towards crizotinib and its reversal by anlotinib in EML4-ALK positive NSCLC

doi: 10.1186/s12967-022-03451-2

Figure Lengend Snippet: CCL20 may induce crizotinib resistance by activation of angiogenesis via JAK2/STAT3-CCL20-VEGFA/IL6 axis. A a) Tube formation of HUVECs cultured in H3122 culture medium (CM), H3122CR CM, H3122 CM supplemented with human recombinant CCL20, and H3122 CM supplemented with rhCCL24. b) Tube formation of HUVECs cultured in the CM of H3122CR transfected with si-negative control (si-NC), si-CCL20, and si-CCL24. The number of tubes and capillary length were analyzed to evaluate angiogenic activity of CCL20 and CCL24; B , C Knockdown of CCL20 suppressed the protein expression of CCL2, IL6, and VEGFA in H3122CR compared with si-NC (Control); D STAT3 was upregulated in H3122CR compared with H3122; E , F Stattic inhibited the JAK2/STAT3 pathway and the protein expression of CCL20 and VEGFA in H3122CR. GAPDH served as a loading control. *p < 0.05, **p < 0.01, ***p < 0.001, ns not significant

Article Snippet: CCL2 (SinoBiological, KIT10134), CCL20 (Abcam, ab269562), and CCL24 (Abcam, ab10050) levels in culture supernatant after treatment for 48 h were measured using ELISA kits.

Techniques: Activation Assay, Cell Culture, Recombinant, Transfection, Negative Control, Activity Assay, Expressing

The inhibition effect of anlotinib on ALK -positive cell lines. A Dose response curve and half maximal inhibitory concentration (IC50) of anlotinib in H2228, H3122, and H3122CR; B Effect of anlotinib treatment on cell colony formation in H2228, H3122, and H3122CR; C Anlotinib inhibited the mRNA and protein expression of CCL20, CCL24, CCL2, and CX3CL1 in H3122CR; D Effect of anlotinib treatment on chemokines expression in H3122 and H2228; E Anlotinib inhibited the JAK2/STAT3-VEGFA/IL6 axis. GAPDH served as a loading control. * p < 0.05, ** p < 0.01, *** p < 0.001, ns: not significant

Journal: Journal of Translational Medicine

Article Title: Role of chemokine-mediated angiogenesis in resistance towards crizotinib and its reversal by anlotinib in EML4-ALK positive NSCLC

doi: 10.1186/s12967-022-03451-2

Figure Lengend Snippet: The inhibition effect of anlotinib on ALK -positive cell lines. A Dose response curve and half maximal inhibitory concentration (IC50) of anlotinib in H2228, H3122, and H3122CR; B Effect of anlotinib treatment on cell colony formation in H2228, H3122, and H3122CR; C Anlotinib inhibited the mRNA and protein expression of CCL20, CCL24, CCL2, and CX3CL1 in H3122CR; D Effect of anlotinib treatment on chemokines expression in H3122 and H2228; E Anlotinib inhibited the JAK2/STAT3-VEGFA/IL6 axis. GAPDH served as a loading control. * p < 0.05, ** p < 0.01, *** p < 0.001, ns: not significant

Article Snippet: CCL2 (SinoBiological, KIT10134), CCL20 (Abcam, ab269562), and CCL24 (Abcam, ab10050) levels in culture supernatant after treatment for 48 h were measured using ELISA kits.

Techniques: Inhibition, Concentration Assay, Expressing